The study explores how glycosaminoglycans (GAGs) influence the angiogenic properties of type I collagen-based scaffolds. Researchers incorporated GAGs into collagen scaffolds and used the IKOSA CAM Assay and Network Formation Assay to evaluate vascular network...
CAM
Artificial Intelligence (AI) Based Analysis of In Vivo Polymers and Collagen Scaffolds Inducing Vascularization
The study evaluates the vascularization potential of two collagen-based scaffolds (2D and 3D structures) and a non-collagen scaffold implanted on the chick embryo chorioallantoic membrane (CAM). The researchers utilized classical stereomicroscopic imaging enhanced...
Analysis of Osteosarcoma Cell Lines and Patient Tissue Using a 3D In Vivo Tumor Model—Possible Effects of Punicalagin
In this study, researchers investigated the potential of punicalagin as a supplementary treatment for osteosarcomas, the most prevalent primary malignant bone tumors affecting children, adolescents, and young adults. Despite current therapies like surgery and...
A Conversation with Dr. Sarah Barnett About Ethical Standards and AI Image Analysis in CAM models
Welcome everybody to today’s interview. The topic today will be “Bridging Science and Ethics: The Evolution of CAM Models, Ethical Standards, and AI Advancements”. Elisa and I are very excited to be speaking with Sarah Barnett from the University of Liverpool today....
Inspiring Hope: How The 3Rs Framework and the CAM Assay Model Set New Standards in Animal Testing
The growing public concern about the use of animals in research causes scholars to reevaluate and optimize existing experimental models. This has led to the rise of the 3Rs concept in animal testing. Thus, alternative in vivo and in vitro models fully replacing...
Isolation and Characterization of ZK002, a Novel Dual Function Snake Venom Protein from Deinagkistrodon Acutus with Anti-Angiogenic and Anti-Inflammatory Properties
ZK002 is a novel protein isolated from the venom of the snake Deinagkistrodon acutus. It has been shown to have both anti-angiogenic and anti-inflammatory activities in both in vitro and in vivo studies. Mechanistically, ZK002 inhibits activation of VEGF signaling and...
A Sustainable, Green-Processed, Ag-Nanoparticle-Incorporated Eggshell-Derived Biomaterial for Wound-Healing Applications
Eggshell membrane (ESM) incorporated with silver nanoparticles (AgNPs) was developed as a promising wound-dressing material with enhanced antimicrobial, biological, and physical properties. Green-synthesized AgNPs displayed greater antimicrobial efficacy and stability...
IKOSA Prisma in Action: Assessing Angiogenesis Hallmarks Within the CAM Assay Model
We present to you a simple AI-driven method to analyze image data collected from the CAM assay model. Bear with us as we will explain to you how to effortlessly automate the entire image analysis process with the help of the IKOSA CAM Assay Application. Table of...
Toxicological Analysis by Assessment of Vascularization and Cell Viability Using the Chicken’s Chorioallantoic Membrane (CAM Assay)
This paper presents a promising alternative in animal experimentation - the CAM assay using hen's eggs. The CAM provides a suitable environment for xenograft implantation, enabling the growth of human tumors. Evaluating therapeutic agents for efficacy and toxicity...
The CAM Model—Q&A with Experts
The chorioallantoic membrane (CAM) model is an alternative in vivo test system for studying cancer hallmarks, such as angiogenesis, tumor growth, immune escape, metastasis, and drug resistance. It is suitable for tumor cell lines and patient-derived xenografts (PDX),...
The Chick Embryo Xenograft Model for Malignant Pleural Mesothelioma: A Cost and Time Efficient 3Rs Model for Drug Target Evaluation
Malignant pleural mesothelioma is a cancer of the lung lining, normally caused by asbestos, that develops decades after exposure. Chemotherapy, and recently more targeted drugs, show some benefit although only a minority of patients respond and invariably the cancer...
IKOSA® CAM Assay Application to Quantify Blood Vessels on Chick Chorioallantoic Membrane (CAM)
Among in vivo angiogenesis assays, chick chorioallantoic membrane (CAM) assay has allowed meaningful progress in elucidating the mechanism of the vasoproliferative response to several pro- and antiangiogenic factors, thanks to its low costs, relatively easy...
A Novel Artificial Intelligence-Based Approach for Quantitative Assessment of Angiogenesis in the Ex Ovo CAM Model
Angiogenesis is a highly regulated process. It promotes tissue regeneration and contributes to tumor growth. Existing therapeutic concepts interfere with different steps of angiogenesis. The quantification of the vasculature is of crucial importance for research on...
A 3D In Vivo Model for Studying Human Renal Cystic Tissue and Mouse Kidney Slices
(1) Background: Autosomal dominant polycystic kidney disease (ADPKD) is a frequent monogenic disorder that leads to progressive renal cyst growth and renal failure. Strategies to inhibit cyst growth in non-human cyst models have often failed in clinical trials. There...
Deep Learning-Based Image Analysis for the Quantification of Tumor-Induced Angiogenesis in the 3D In Vivo Tumor Model—Establishment and Addition to Laser Speckle Contrast Imaging (LSCI)
(1) Background: angiogenesis plays an important role in the growth and metastasis of tumors. We established the CAM assay application, an image analysis software of the IKOSA platform by KML Vision, for the quantification of blood vessels with the in ovo...
Hyaluronic Acid with Bone Substitutes Enhance Angiogenesis In Vivo
The study evaluated the pro-angiogenic properties of a commercially available bovine bone-substitute material with hyaluronic acid (BSM+) in comparison to the same bone-substitute material without hyaluronic acid (BSM) in an in-vivo model. BSM+ and BSM were incubated...
Zinc Oxide Nanoparticles Exhibit Favorable Properties to Promote Tissue Integration of Biomaterials
Zinc oxide nanoparticles (ZnO NPs) are a promising material for tissue engineering applications. They have a wide range of biocompatibility, antibacterial properties, and can be modified to have different shapes, sizes, and functions. In a recent study, researchers...
The Uses of CAM Assay Angiogenesis Analysis Software
As the chick chorioallantoic membrane (CAM) model raises in popularity in scientific literature, many researchers express interest in using automated bioimage analysis software tailored to the specifics of the CAM method. Our team...
The Promise of AI in Angiogenesis Quantification
In this article, we introduce five angiogenesis quantification software solutions that will help you harness the potential of AI-driven image analysis in vasculogenesis research. Find out how state-of-the-art computer vision technology will assist you in the study of...
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