The study evaluates the vascularization potential of two collagen-based scaffolds (2D and 3D structures) and a non-collagen scaffold implanted on the chick embryo chorioallantoic membrane (CAM).
The researchers utilized classical stereomicroscopic imaging enhanced with IKOSA software, employing the CAM assay and the Network Formation Assay to assess vessel branching potential, vascular area, tube length, and thickness.
Findings indicated that both collagen-based scaffolds promoted non-inflammatory angiogenesis. In contrast, the non-collagen scaffold induced significant inflammation, leading to inflammation-related angiogenesis. Notably, the 3D collagen scaffold demonstrated a sustained and continuous angiogenic process, evidenced by an exponential increase in vessel branching points and total vascular area until day 5 of the experiment.
The study concludes that collagen-based scaffolds, particularly those with a 3D structure, may be more suitable for neovascularization compared to non-collagen scaffolds. Additionally, the research highlights the potential of combining the CAM model with AI-based software for evaluating vascularization in biomaterials, offering a promising approach to reduce and replace animal experimentation in biomaterial pre-screening.
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