Periosteum-derived micrografts show strong potential to enhance vascularization when combined with commonly used bone substitute materials. In this study, micrografts containing mesenchymal stem cell-like cell populations were evaluated in the chorioallantoic membrane (CAM) model together with several clinically established scaffolds. The addition of micrografts significantly increased vascular parameters such as vessel area, vessel length, and branching points compared with scaffold-only controls in multiple groups, indicating improved angiogenic activity. Histological analyses and SMA staining further supported the presence of denser and more mature vascular structures in the treated groups.
Quantitative evaluation of angiogenesis was performed using the IKOSA CAM Assay, enabling standardized measurement of vascular growth on the CAM surface. IKOSA AI was further used to analyze OCT and SMA images, providing automated and reproducible quantification of vessel number, vessel area, and related parameters.
Importantly, the study reports that IKOSA-based analysis outperformed traditional manual counting in precision when evaluating branching points and vascular thickness, and enabled the assessment of additional vascular parameters that cannot be reliably measured using manual methods. These findings highlight the value of IKOSA workflows for precise and consistent preclinical angiogenesis assessment in regenerative biomaterial research.
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